The major constituent of the shampoo is sodium lauryl sulphate which imparts cleansing or detergency effect to shampoo. This crucial agent causes irritation to skin and eyes and precipitate toxic effects in users. Therefore, it is indispensable to determine its contents in the shampoo. A simple and rapid spectrophotometric method for the determination of sodium lauryl sulphate (SLS) is employed in this study. SLS forms a coloured complex with methylene blue dye. The complex is extracted in chloroform. The absorbance of complex in chloroform layer is measured at maximum wavelength (max) of 653 nm. The calibration curve is linear in the range of 2-20 ppm of SLS concentration. On the basis of the linearity curve the SLS concentration in marketed shampoos is evaluated. The evaluated concentration is compared with US FDA specification limit of SLS content in shampoo.
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